Application of next-generation sequencing technology in forensic science / 中国法医学杂志

With the rapid development of Next-generation Sequencing(NGS)technologies, and its high throughput and low cost is applied widely in the field of life science, the increase in the depth of sequencing together decrease in the consumption of time and cost, makes a wide application of NGS in the research of microbiological research, ancient DNA study, clinical diagnosis, forensic science research, etc. The article discusses the second generation sequencing technology platform and its genetic markers in the forensic application. Included STR typing, SNP typing, HLA genotype prediction and the application in the degradation of the material.

Species Specificity of Male Human's 29 Y-SNP Loci / 昆明医科大学学报

Objective To explore species specificity of 29 Y-SNP loci and to lay the foundation for forensic application.Methods Human DNA and 8 different kinds of common animals' DNA were amplified separately by PCR.The PCR products were analyzed with PAGE.Results Twenty-three out of 29 Y-SNP loci were only amplified by the male human DNA,which indicated a good species specificity.Three loci amplified from human and some animals showed that the length of the product was different between two species.Another three loci amplified from human and animal showed that the length of the fragment was similar between human and animal.Five loci were amplified by PCR products from both male and female samples.Conclusion Most of 29 Y-SNP loci,amplified in male humans,have good species specificity and can be directly used for personal identification and paternity testing.

Polymorphism Investigation on 15 STR Loci of Yunnan Bai Population / 昆明医科大学学报

Objective The study aimed to investigate 15 autosomal loci polymorphism(such as D3S1358 and TPOX)in Nujiang Bai nationality,in order to construct genetic basic data of Bai population and provide a foundation for population study and forensic cases. Methods Venous blood was collected from 124 unrelated Bai individuals in Nujiang,Yunnan province. DNA was extracted by classical organic solvent extraction. PCR technique was applied to amplify the sequence of autosomal loci. The products were measured by electrical fluoroscopy. The gene frequencies of 15 STR loci were investigated and genetic polymorphisms were analyzed. ResultsGene polymorphisms of 15 STR loci were detected and the distributions of genotype conformed with Hardy-Weinberg balance law(P > 0.05). The cumulative match probability(CPM)was 4.869×10-17,the cumulative excluding probability(CPE)was 99.999 99%,and the combined power of discrimination(CDP)was 99.999 97%. ConclusionThe 15 STR loci used in this study were highly polymorphic informative content in Bai population and could be applied to population study and forensic practice.

Practice and Experience of Tutorial System for Undergraduate in Forensic Biology Teaching / 昆明医科大学学报

To introduce tutorial system for undergraduate in the forensic biology teaching is an important measure of educational reform of forensic medicine.The article presented some schemes and experiences about the practice of tutorial system for undergraduate in the forensic biology department of Kunming Medical College.

Exploration on the Experimental Teaching Reform of Forensic Biology / 昆明医科大学学报

Based on the experience of experimental teaching reform of forensic biology,the article made a systematic exposition about the measures and experience of the research and practice of the reform from the following aspects: aim of the reform,priority of the reform and specific measures.By reforming experimental teaching methods,the students' interests and performance record have been significantly improved.

Quality Control Standard and Open Management Mode of Forensic Biology DNA Laboratory / 昆明医科大学学报

The article elaborated the standard of quality control,open service management system and requirements for system construction of forensic biology laboratory,which is to share laboratory recourses and advance the healthy and sustainable development of the laboratory.

Summary of the Reform of Course Content and Methods of Forensic Biology / 昆明医科大学学报

Forensic biology is a main subject of forensic medicine.With the rapid development of science and technology,the courses should be constantly improved.New teaching mode has come into being application of multimedia technology in theoretical lessons,case teaching,independent completion of experimental lessons,which is thanks to the constant reinforcement of teaching staff and the reform of course content and teaching methods.

Discussion on the Operational Mechanism of Forensic Biology Laboratory / 昆明医科大学学报

The reform for the laboratory operational mechanism includes laboratory management system reform and experimental teaching reform.The author made some explorations and research to the operational mechanism and management pattern of university laboratory of forensic biology,then presented some ideas.

The Detection of RhD? pseudogene and RhD-CE-D Hybridization Gene and Medicine Application in Rh-negative ones of Kunming Area / 医学研究杂志

Objective To study the D gene polymorphism of Rh-negative blood group in Kunming resident population with PCR technology for gene typing,and to establish and perfect PCR detecting method for RhD gene.Method 46 samples of Rh-negative blood samples were collected and PCR amplification with exon-SSP had been done firstly.The samples of D gene part existing were screened,then PCR amplifying by intron-SSP had been conducted.The PCR amplification production with intron-SSP was used to DNA sequence.Results Among 46 Rh-negative samples,the number of D gene lost intacfly was 29;the complete existing one with all exons was 12.The sequencing results were that 2 samples of only containing D10 were RhD-CE(3-9)-D and the sample with only D4 and D6 was the newly discovered variant allele RhD-CE(2-3,5,7-9)-D.RhD ? was not found in our study.Conclusion(1)There is high frequency of RhD-CE(3-9)-D in Kunming area.(2)A new variant allele of RhD-CE(2-3,5,7-9)-D is first found in Kunming area.